Detection and characterization of receptor interactions with PDZ domains.
نویسندگان
چکیده
Many transmembrane receptors are regulated by associations with scaffold proteins containing PDZ domains, which interact with receptor carboxyl-termini to control receptor signaling, trafficking, and localization. We describe here approaches for detecting and characterizing interactions between receptors and PDZ scaffolds. These approaches include the construction and screening of proteomic arrays, blot overlays using fusion proteins, and co-immunoprecipitation studies to assess interactions in cells.
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ورودعنوان ژورنال:
- Methods in molecular biology
دوره 756 شماره
صفحات -
تاریخ انتشار 2011